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pinfuse migg2b fc2 vector  (InvivoGen)


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    Structured Review

    InvivoGen pinfuse migg2b fc2 vector
    Pinfuse Migg2b Fc2 Vector, supplied by InvivoGen, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pinfuse+migg2b+fc2+vector/pINFUSE-mIgG2B-Fc2/pm41110474-478-44-46
    Average 93 stars, based on 9 article reviews
    pinfuse migg2b fc2 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: Novel single-domain antibodies targeting a unique transferrin receptor 1 epitope for cross-species delivery of drugs in the central nervous system.
    Article Snippet: The blood-brain barrier (BBB) is a major obstacle for delivering therapeutic agents to the central nervous system (CNS), posing significant challenges for treating neurological disorders.. Among current strategies to improve brain drug exposure, hijacking physiological pathways involved in receptor-mediated transcytosis has emerged as a promising strategy.. While targeting transferrin receptor 1 (TfR1) is widely explored, many TfR1antibodies lack cross-species reactivity, limiting translational development.

    Article Title: Generation of a novel therapeutic peptide that depletes MDSC in tumor-bearing mice
    Article Snippet: FACS analysis was performed using LSRFortessa cell analyzer (BD Biosciences) and results were analyzed using FlowJo software. .. Synthetic, complementary double-stranded oligo nucleotides encoding H6 or G3 peptide were fused with a human IL-2 signal peptide and 6 × His tag and then cloned into EcoRI and BglII sites of pINFUSE-mIgG2b-Fc2 vector (InvivoGen, San Diego, CA). .. For initial characterization, we produced recombinant peptibodies by transfecting 293T human embryonic kidney cells with the plasmid constructs using Lipofectamine 2000 kit (Life Technologies, Grand Island, NY).

    Article Title: TFF3 is a ligand for LINGO2 that de-represses EGFR to control disease outcome during colitis and gastrointestinal nematode infection
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths.
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier
    Article Snippet: .. Cloning of mouse, human and rhesus monkey TfR1 ectodomains in fusion with a mouse IgG2a Fc cDNAs encoding the mouse, human and rhesus monkey TfR1 ectodomains (amino acids 89 to 763 from mouse TfR1 (NP_001344227.1), amino acids 89 to 760 of the human TfR1 (AAA61153.1) and rhesus monkey TfR1 (NP_001244232.1)) were obtained by gene synthesis (Genecust) and cloned in the pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to encode a mouse IgG2a Fc (mFc) fused in C-terminus to the mouse, human and rhesus monkey TfR1 ectodomains. ..

    Plasmid Preparation:

    Article Title: TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: Novel teleost CD4-bearing cell populations provide insights into the evolutionary origins and primordial roles of CD4 + lymphocytes and CD4 + macrophages
    Article Snippet: The expression of CD4-1, CD4-2a, CD4-2b, and LAG-3 on NRK cells was confirmed by flow cytometry with a murine anti-HA mAb (Sigma-Aldrich). .. With the goal to produce soluble CD4-2b, the cDNA sequence encoding the Ig domains of CD4-2b were also subcloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen). .. CD4-2b/Fc fusion protein was produced with FreeStyle 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s instructions, and then purified with HighTrap Protein G HP column (GE Healthcare).

    Article Title: Novel single-domain antibodies targeting a unique transferrin receptor 1 epitope for cross-species delivery of drugs in the central nervous system.
    Article Snippet: The blood-brain barrier (BBB) is a major obstacle for delivering therapeutic agents to the central nervous system (CNS), posing significant challenges for treating neurological disorders.. Among current strategies to improve brain drug exposure, hijacking physiological pathways involved in receptor-mediated transcytosis has emerged as a promising strategy.. While targeting transferrin receptor 1 (TfR1) is widely explored, many TfR1antibodies lack cross-species reactivity, limiting translational development.

    Article Title: Generation of a novel therapeutic peptide that depletes MDSC in tumor-bearing mice
    Article Snippet: FACS analysis was performed using LSRFortessa cell analyzer (BD Biosciences) and results were analyzed using FlowJo software. .. Synthetic, complementary double-stranded oligo nucleotides encoding H6 or G3 peptide were fused with a human IL-2 signal peptide and 6 × His tag and then cloned into EcoRI and BglII sites of pINFUSE-mIgG2b-Fc2 vector (InvivoGen, San Diego, CA). .. For initial characterization, we produced recombinant peptibodies by transfecting 293T human embryonic kidney cells with the plasmid constructs using Lipofectamine 2000 kit (Life Technologies, Grand Island, NY).

    Article Title: TFF3 is a ligand for LINGO2 that de-represses EGFR to control disease outcome during colitis and gastrointestinal nematode infection
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths.
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier
    Article Snippet: .. Cloning of mouse, human and rhesus monkey TfR1 ectodomains in fusion with a mouse IgG2a Fc cDNAs encoding the mouse, human and rhesus monkey TfR1 ectodomains (amino acids 89 to 763 from mouse TfR1 (NP_001344227.1), amino acids 89 to 760 of the human TfR1 (AAA61153.1) and rhesus monkey TfR1 (NP_001244232.1)) were obtained by gene synthesis (Genecust) and cloned in the pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to encode a mouse IgG2a Fc (mFc) fused in C-terminus to the mouse, human and rhesus monkey TfR1 ectodomains. ..

    Construct:

    Article Title: TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: TFF3 is a ligand for LINGO2 that de-represses EGFR to control disease outcome during colitis and gastrointestinal nematode infection
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Article Title: TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths.
    Article Snippet: The digested TFF3 PCR product with HindIII and Xho enzymes was cloned into pcDNA3:mRFP vector (Addgene) to generate pcDNA3:mTFF3:RFP construct. .. Using the similar method, the full-length mTFF3 coding region was also cloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to generate pINFUSE-TFF3:mIgG2b-Fc2 construct. .. The full-length mCXCR4 coding region was amplified with mCXCR4 forward primer with HindIII overhang and mCXCR4 reverse primer with XhoI overhang using mCXCR4 cDNA plasmid (OMu23034D, Origene) as the template.

    Sequencing:

    Article Title: Novel teleost CD4-bearing cell populations provide insights into the evolutionary origins and primordial roles of CD4 + lymphocytes and CD4 + macrophages
    Article Snippet: The expression of CD4-1, CD4-2a, CD4-2b, and LAG-3 on NRK cells was confirmed by flow cytometry with a murine anti-HA mAb (Sigma-Aldrich). .. With the goal to produce soluble CD4-2b, the cDNA sequence encoding the Ig domains of CD4-2b were also subcloned into pINFUSE-mIgG2b-Fc2 vector (InvivoGen). .. CD4-2b/Fc fusion protein was produced with FreeStyle 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s instructions, and then purified with HighTrap Protein G HP column (GE Healthcare).

    Cloning:

    Article Title: Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier
    Article Snippet: .. Cloning of mouse, human and rhesus monkey TfR1 ectodomains in fusion with a mouse IgG2a Fc cDNAs encoding the mouse, human and rhesus monkey TfR1 ectodomains (amino acids 89 to 763 from mouse TfR1 (NP_001344227.1), amino acids 89 to 760 of the human TfR1 (AAA61153.1) and rhesus monkey TfR1 (NP_001244232.1)) were obtained by gene synthesis (Genecust) and cloned in the pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to encode a mouse IgG2a Fc (mFc) fused in C-terminus to the mouse, human and rhesus monkey TfR1 ectodomains. ..



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